Long PCR
Definition of Long PCR:
Long PCR is a PCR is which extended or longer than standard PCR, meaning over 5 kilobases (frequently over 10 kb). Long PCR is usually only useful if it is accurate. Thus, special mixtures of proficient polymerases along with accurate polymerases such as Pfu are often mixed together.
Applications of Long PCR
Long PCR is often used to clone larger genes or large segments of DNA which standard PCR cannot.
External Links to Long PCR Articles
Long PCR Reagents and Guidelines
See LA PCR (Long and Accurate PCR)
Long PCR Protocols
Optimization and Tips for Long PCR
Long PCR Amplification Procedure
Long PCR Recently Published Research
Aqueous suspension of carbon nanotubes enhances the specificity of long PCR. Related Articles
Aqueous suspension of carbon nanotubes enhances the specificity of long PCR.
Biotechniques. 2008 Apr;44(4):537-8, 540, 542, passim
Authors: Zhang Z, Shen C, Wang M, Han H, Cao X
DNA manipulation technology is facing more challenges in the postgenomics era. More and more nanomaterials have been investigated for their potential implications in developing better gene technology. In this study, we reported the beneficial effect of single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) in enhancing the specificity and total efficiency of long (14 kb) PCR. Hydroxylic and carboxylic carbon nanotubes (CNTs) had similar enhancing effects. Nanotubes could become another component for improvements in the amplification of long DNA.
PMID: 18476818 [PubMed - in process]