PCR Mutagenesis
PCR Mutagenesis
PCR can be used to make changes to the nucleotide sequence of DNA, this is called PCR Mutagenesis. This method can be utilized to assess the function of a promoter, and to alter amino acids to test the functions of domains in a protein. Mutations can be introduced in PCR by several methods.
Site-Directed Mutagenesis PCR
Site-directed mutagenesis allows one to introduce a mutation at a specific location on the DNA strand. This is accomplished by using primers for the PCR which are altered at one or more nucleotides.
Random Mutagenesis PCR
This PCR method is based on the use of error-prone polymerase which lack 3'-5' exonuclease activity. Using these polymerases, one can induce random mutations along the pcr products.