Bacterial Colony PCR. Karl Clark, Regents of the University of Minnesota. This colony protocol allows for the rapid detection of transformation success when primers are available to allow determination of correct ligation products by size or hybridization. - [More about Bacterial Colony PCR Procedure]

Colony PCR is a useful technique to determine whether or not a specific colony on a plate has a sequence which is desired. Primers for the specific sequence should be used when preparing the reaction cocktail. Examples of primers for colony pcr include: antibiotic resistance specific primers, or primers flanking a cloned region. (per Adam 8/12/04). Univ. Chicago. The Preuss Lab. - [More about Colony PCR]

Colony PCR - Procedure. Maddock Lab. - [More about Colony PCR - Procedure]

Protocol in Yeast S. Pombe colonies. Colony PCR can identify colonies of yeast where your target gene has been replaced with a marker gene by homologous recombination, and also to distinguish homologous recombination events from non-homologous. Forsburg Lab. - [More about Colony PCR in Pombe]

Colony PCR protocol designed to quickly screen for plasmid inserts directly from E. coli colonies. Very nice and detailed protocol. Michael L. Summers, Ph.D., Department of Biology, California State University Northridge. - [More about Colony PCR Methodology - PDF]

Colony PCR OpenWetWare. Decent short protocols for Colony PCR. - [More about Colony PCR OpenWetWare]

The colony pcr procedure will work for both yeast and E. coli. David Amberg, Department of Biochemistry and Molecular Biology. SUNY Upstate Medical University. Simple protocol with colony PCR Conditions. - [More about Colony PCR Protocol]

E. coli Colony PCR. Very simple Protocol. Aris Lab. Department of Anatomy and Cell Biology University of Florida Health Science Center. - [More about E.Coli Colony PCR - PDF]

Colony PCR Protocol, contributed by Lynn Hancock. - [More about Enterococcus Colony PCR]

Single tube confirmation PCR protocol for Yeast. yeast deletion project. Stanford. - [More about Single tube confirmation PCR protocol for Yeast]

Based on Akada et al., Biotechniques 28:668-674 (April 2000). Fred Hutchinson Cancer Research Center. Seattle, WA. Very detailed protocol for Yeast Colony PCR. - [More about Yeast Colony PCR]

Breeden Lab. Fred Hutchinson Cancer Research Center. Seattle, WA. Buffers, colony PCR mastermix, and procedure for colony pcr from yeast colonies. - [More about Yeast colony PCR (analytic) protocol]