In Situ PCR
In Situ Polymerase Chain Reaction, ISH PCR, in situ PCR. Methods and Protocols for In situ polymerase chain reaction.
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Radioactive 3H-labeled nucleotides permit an
excellent distinction with colorimetric signals for co-localization, although two distinct
chromogens can in many instances allow successful localization of two different targets.
PCR RT in situ PCR. Gerard J. Nuovo. Volume 49(11): 1329–1339, 2001. The Journal of Histochemistry & Cytochemistry - [More about Co-labeling Using In Situ PCR: A Review PDF]
Improvements in Fluorescent In Situ RT-PCR for Medical and Research Use. Robert Bacallao, M.D. et al. Department of Medicine, Section of Nephrology
Indiana University School of Medicine. A short summary of In situ PCR including: Description, Potential Areas of Application, Main Advantages of this Invention, State of Development, Intellectual Property Status. - [More about Improvements in Fluorescent In Situ RT-PCR for Medical and Research Use]
In situ PCR. Past, Present, Future. Dr. med. Paul Komminoth. PCR in situ or PCR in situ hybridization. Pitfalls and Limitations, applications, Future Aspects. A very good review. Roche. - [More about In Situ Polymerase Chain Reaction:]
In Situ PCR on Plant Material. Bo Johansen. In situ PCR - Required materials, Required solutions, Tissue preparation, Pectinase treatment, Protease treatment, Acetylation, DNase treatment, Reverse transcription, Thermal cycling, Detection
Mounting. - [More about In Situ PCR on Plant Material]
Technical Aspects/Philosophy, In Situ PCR Primer Design, Reaction Conditions, "Mechanics" of the Reaction, Methodology, Detection of Amplified DNA by in situ Hybridization, In Situ Amplification, for cells and tissues. Includes detailed protocols. Ernest F. Retzel, Katherine A. Staskus, Janet E. Embretson and Ashley T. Haase
Department of Microbiology, University of Minnesota, Minneapolis - [More about The In Situ PCR: Amplification and Detection in a Cellular Context]