Allele-Specific PCR Alel-specifično PCR
Allele-Specific PCR Protocol Alel specifični protokol PCR
Allele-specific PCR is a varation of the polymerase chain reaction which is used as a diagnostic or cloning technique, to identify or utilize single-nucleotide polymorphisms (SNPs) (single base differences in DNA). Alel-specifično PCR je varation od verižna reakcija s polimerazo, ki se uporablja kot diagnostični ali kloniranje tehniko, katerih namen je identificirati ali z enim naročiti pri nukleotidno polimorfizmov (SNPs) (enotne osnove razlik v DNK). Allele-specific PCR does require the sequence of the target DNA sequence, including differences between the alleles. Alel-specifično PCR ne zahtevajo zaporedje ciljnega zaporedja DNA, vključno z razlikami med alelov.
Primers for Allele-Specific PCR Oligonukleotidov za alel-Posebni PCR
The allele-specific PCR uses primers whose 3' ends encompass the SNP. Alel specifične PCR primerji uporab, katerih 3 'koncih obsega SNP.
PCR amplification under stringent conditions is much less efficient in the presence of a mismatch between template and primer, so successful amplification with a SNP-specific primer signals presence of the specific SNP in a sequence. PCR amplification pod strogimi pogoji je precej manj učinkovito v prisotnosti neusklajenost med predlogo in primer, da uspešno ojačanja s SNP-posebnih signalov Osnovni prisotnost posebnega SNP v zaporedju.
Allele-Specific PCR References Alel-specifično PCR Literatura
Saiki RK, Bugawan TL, Horn GT, Mullis KB, Erlich HA (1986). Saiki RK, Bugawan TL, Horn GT, Mullis KB, Erlich HA (1986). "Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes.". Nature 324 : 163-166. PMID 3785382 . "Analiza encimsko dopolnjena beta-globin in HLA-Dq alfa DNA z alel-specifično oligonukleotid sonde.". Nature 324: 163-166. PMID 3.785.382.