Assembly PCR Skupščine PCR
Assembly PCR Protocol Skupščine protokol PCR
Assembly PCR is a polymerase chain reaction variation that artificial synthesizes long DNA sequences by performing PCR on a pool of long oligonucleotides (primers) with short overlapping segments. Skupščine PCR je verižna reakcija s polimerazo umetna synthesizes sprememba, ki je dolga zaporedja DNK, ki jih opravlja PCR na bazen dolgih oligonucleotides (oligonukleotidov) s kratkimi prekrivajočih se segmentih.
The oligonucleotides alternate between sense and antisense directions, and the overlapping segments determine the order of the PCR fragments thereby selectively producing the final long DNA product. V oligonucleotides namestnika med smisel in protismiselni smereh, in prekrivanja segmentov določi, da o tem fragmente PCR selektivno proizvajajo končni izdelek, dolgo DNK.
Assembly PCR References Skupščine PCR Literatura
Stemmer WP, Crameri A, Ha KD, Brennan TM, Heyneker HL (1995). Stemmer WP, Crameri A, Ha KD, Brennan TM, Heyneker HL (1995). "Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides". Gene 164 : 49-53. PMID 7590320 . "Single-korak je sestavljen iz gena in celoten plazmid iz velikega števila oligodeoxyribonucleotides". Gene 164: 49-53. PMID 7.590.320.