PCR Mutagenesis PCR Mutagenesis
PCR Mutagenesis PCR Mutagenesis
PCR can be used to make changes to the nucleotide sequence of DNA, this is called PCR Mutagenesis. PCR se lahko uporabi do sprememb nukleotidnega zaporedja DNK, to se imenuje PCR Mutageneza. This method can be utilized to assess the function of a promoter, and to alter amino acids to test the functions of domains in a protein. Ta metoda se lahko uporabljeni za oceno delovanja promotor, in naj ustrezno spremeni aminokisline za preskus področja nalog na beljakovine. Mutations can be introduced in PCR by several methods. Mutacij se lahko uvedejo v PCR z več metodami.
Site-Directed Mutagenesis PCR Site-usmerjena mutageneza PCR
Site-directed mutagenesis allows one to introduce a mutation at a specific location on the DNA strand. Site-usmerjene mutageneze omogoča uvedbo mutacije na specifični lokaciji na verige DNA. This is accomplished by using primers for the PCR which are altered at one or more nucleotides. To se izvede z uporabo oligonukleotidov za PCR, ki se spremenijo na enega ali več nukleotidov.
Random Mutagenesis PCR Naključne mutageneze PCR
This PCR method is based on the use of error-prone polymerase which lack 3'-5' exonuclease activity. Ta metoda je PCR, ki temelji na uporabi error-polimeraza, ki so nagnjene pomanjkanja 3'-5 'exonuclease dejavnost. Using these polymerases, one can induce random mutations along the pcr products. Uporaba teh polimeraze, lahko povzročijo naključno mutacij vzdolž produktov PCR.