Assembly PCR Pagtitipon ng PCR
Assembly PCR Protocol Kapulungan PCR Protocol
Assembly PCR is a polymerase chain reaction variation that artificial synthesizes long DNA sequences by performing PCR on a pool of long oligonucleotides (primers) with short overlapping segments. Kapulungan PCR ay isang polymerase chain reaction ng pagkakaiba-iba na artipisyal synthesizes long DNA sequences sa pamamagitan ng pagpapalabas na PCR sa isang pool ng mahaba oligonucleotides (primers) sa maikling magkasanib-sanib segment.
The oligonucleotides alternate between sense and antisense directions, and the overlapping segments determine the order of the PCR fragments thereby selectively producing the final long DNA product. Ang oligonucleotides sa pagitan ng mga alternatibong kahulugan at antisense direksyon, at ang magkasanib-sanib segment matukoy ang mga order ng PCR fragments sa gayon selectively paggawa ng huling long DNA produkto.
Assembly PCR References Kapulungan PCR References
Stemmer WP, Crameri A, Ha KD, Brennan TM, Heyneker HL (1995). Stemmer WP, Crameri A, Ha KD, Brennan TM, Heyneker HL (1995). "Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides". Gene 164 : 49-53. PMID 7590320 . "Single-hakbang na kapulungan ng isang gene at buong plasmid mula sa malaking bilang ng mga oligodeoxyribonucleotides". Gene 164: 49-53. PMID 7590320.