PCR Assembly PCR大会聚合酶链反应

Assembly PCR Protocol聚合酶链反应议定书大会

Assembly PCR is a polymerase chain reaction variation that artificial synthesizes long DNA sequences by performing PCR on a pool of long oligonucleotides (primers) with short overlapping segments.大会PCR是一种聚合酶链反应的变化,人工合成的DNA序列的PCR技术表演池长义寡核苷酸(引)短重叠的部分。

The oligonucleotides alternate between sense and antisense directions, and the overlapping segments determine the order of the PCR fragments thereby selectively producing the final long DNA product.候补的寡核苷酸之间的正义和反义的方向,和重叠部分确定的顺序的PCR片段从而选择性生产的最终产品长期的DNA 。

Assembly PCR References聚合酶链反应参考大会

Stemmer WP, Crameri A, Ha KD, Brennan TM, Heyneker HL (1995).施特默尔可湿性粉剂, Crameri阿,哈科威特第纳尔,布伦南商标, Heyneker HL ( 1995年) 。 "Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides". Gene 164 : 49-53. PMID 7590320 . “单步组装的整个基因质粒从大量的义寡核苷酸。 ” 基因 164 : 49-53 。 PMID 759.032万