Conditions and Factors Affecting PCR条件和影响因素聚合酶链反应
Factors Affecting PCR:聚合酶链反应的影响因素：
PCR Reaction Volume PCR反应体积
If you are increasing the PCR reaction volume proportionally to a PCR reaction that is optimized or works well, in general you will increase the amount of product with increasing volume.如果您是提高PCR反应体积比例的PCR反应是优化或运作良好，一般你会增加产品的数量不断增加。 Exceptions to this include older mineral oil PCR machines and PCR thermocyclers without a heated-lid.例外情况包括老年人矿物油PCR检测机器和聚合酶链反应thermocyclers未经加热盖。 In these cases, increasing the PCR reaction volume will negatively affect the PCR product and efficiency of the reaction.在这种情况下，增加了PCR反应量将产生不利影响的PCR产物和效率的反应。
PCR Primer Design PCR引物设计
Primer are usually chosen to be between 18-25 nucleotides in length.底漆通常要选择18-25个核苷酸之间的长度。 Longer primers also work 30-40 nucleotides or bp during pcr也不再引工作30-40个核苷酸或碱基期间聚合酶链反应
A GC clamp at the 5' ends of the primers helps.气相色谱钳在5 '端的引物有所帮助。
One can thus design primers with 1 or 2 nucleotides GC at the start and ends of the primers if possible因此，人们可以设计引物与1或2个核苷酸的GC在开始和结束的引如果可能的话