Late-PCR

Linear-After-The-Exponential-PCR Protocol

A recent modification of assymetric PCR is Linear-After-The-Exponential-PCR or LATE-PCR.

 

Applications of Asymmetric PCR

The Asymmetric PCR is useful in some sequencing and hybridization probing applications where having only one of the two complementary stands is sufficient or required.

 

Asymmetric PCR Method

The asymmetric PCR method is conducted as the standard pcr protocol, however a great excess of the primers for the chosen strand is used.

Due to the slow (arithmetic) amplification later in the reaction after the limiting primer has been used up, extra cycles of PCR are required.

Also see Assymetric PCR.

 

Primers for LATE-PCR

LATE-PCR utilizes a limiting primer with a higher melting temperature (Tm) than the excess primer to maintain reaction efficiency as the limiting primer concentration decreases mid-reaction.[16]

Troubleshooting LATE-PCR